Gyrolab kits: Bioprocess
Kit convenience. Gyros quality.

Easy-to-use kits require fewer manual steps,
reducing hands-on time and risk for error.

Gyrolab™ CHO-HCP Kit 1

Gyrolab CHO-HCP Kit 1Gyrolab CHO-HCP kit 1 is designed to measure Chinese Hamster Ovary (CHO) Host Cell Proteins (HCP) impurities with performance that addresses many of the limitations of ELISA. The kit, which covers a broad spectrum of HCPs, broadens the analytical range from typically two orders of magnitude to four, simplifying spike recovery and dilution linearity experiments.

Easy to use kit

  • Optimized kit ensures assay robustness, convenience and rapid results
  • No need for assay development

Broad dynamic range

  • Measurement range over 4 logs
  • Minimizes dilutions and repeats

Automation and efficient analysis

  • Reduces hands-on time and risk for errors
  • Reproducible high quality data
  • 96 data points in 75 min

Assay principle

The Gyrolab CHO-HCP sandwich assay is run on Gyrolab Bioaffy 1000HC CD, which is optimized for HCP analysis. The biotinylated anti-HCP antibody, which is captured on streptavidin-coated beads in the affinity column, binds to a broad spectrum of CHO-HCPs. Bound HCP is then detected using a fluorescently labeled anti-HCP antibody. The Gyrolab instrument transfers reagents and samples to the CD and then records the laser-induced fluorescence from each column. Samples are quantitated using a standard curve.

Typical assay data

 

Gyrolab™ Protein A KitGyrolab Protein A Kit

Gyrolab Protein A Kit is designed to detect native and recombinant protein A residuals in samples, such as antibody preparations after Protein A purification processes. In Gyros’ automated method, samples are acidified in the Gyrolab Mixing CD to dissociate protein A-IgG complexes. The amount of Protein A is then determined by a sandwich immunoassay.

  • Quantifies native protein A, recombinant protein A variants and GE Healthcare’s MabSelect SuRe™ ligand
  • Automates acid dissociation
  • Provides results within 80 minutes
  • Delivers precise, accurate data

 Standard assay protocol:

  1. Dilute samples to appropriate IgG concentration
  2. Dilute standards in series to set up seven points of diluted standard between 0.05 ng/ml and 40 ng/ml
  3. Dilute the capture antibody to 100 ug/ml and the detection antibody to 25 nM
  4. Prepare a microtiter plate with standards, capture and detection antibodies, acid dissociation buffers and wash buffers
  5. Load samples, reagents and Gyrolab Mixing CD(s) in a Gyrolab instrument
  6. First results available within 80 minutes

Typical assay data

 

 

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