Vaccine development
Cytokine quantification in small animal models
Studies at the Department of immunology, National Veterinary Institute, Sweden indicated the potential to enhance vaccination against viral infections by using Ginseng as adjuvant.
In an effort to overcome difficulties and delays with ELISA assays, Gyrolab was used to determine cytokine concentrations directly in mice serum samples, as well as cell cultures of re-stimulated spleen cells.
The bar chart shows detection of mouse cytokine IL-10 in serum, in the presence of the five different adjuvants, using Gyrolab. (Detection by ELISA was not successful as dilution to the required working volumes brought cytokine levels below the limit of detection.)
The ability to detect cytokines directly in serum samples with a minimum of sample dilution means that the time required to confirm biological response can be greatly reduced. As such, there is the potential for significant reduction in vaccine development times.
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