
	Protein Quantification

	Overview

	Protein quantification at nanoliter scale

	Single system solution for quantification of monoclonal IgG and host cell proteins

	Increasing efficiency in biopharmaceutical process development

	Pharmacokinetics of therapeutic antibodies

	Quantification of antigen-specific antibodies in serum

	Insulin quantification: save sample and increase efficiency

	Cytokine quantification in small animal models




	Order Application Reports

Insulin quantification: save sample and increase efficiency

Insulin is one of the most extensively studied metabolic factors in diabetes. Current techniques for insulin quantification have limitations in terms of assay performance and throughput. Gyros carried out separate collaborative projects with two groups working with diabetes research.

By working at nanoliter scale, Gyrolab Bioaffy reduced the quantity of sample and reagents required, as well as increasing throughput and reducing 'hands-on' time.

	•		Increased throughput (112 data points delivered in <60 minutes)

	•		Broad measurement range

	•		Reduced cost per data point

	•		Excellent reproducibility

Using Gyrolab Bioaffy, standard curves for rat and human insulin showed a linear range of more than three orders of magnitude. This allows for the quantification of higher concentration samples without the need for sample dilution.

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The results generated by Gyrolab Bioaffy correlated well with the established techniques, with the additional advantage of using smaller sample volumes.

The table shows a comparison of the RIA and ELISA (Karolinska Institutet), ELISA (Novo Nordisk) and Gyrolab Bioaffy techniques used in the two studies.

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The graph shows the correlation between Gyrolab Bioaffy, ELISA, and RIA when quantifying human insulin in serum.

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Gyrolab Bioaffy generated excellent recovery data from plasma, serum and buffer samples spiked with insulin-Asp, indicating that there are no significant matrix effects when using the system.

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Gyrolab Bioaffy along with Gyrolab Workstation LIF reduced the overall analysis time and the amount of 'hands-on' time required compared with the commercial ELISA technique.

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Results courtesy of Diabetes Center Karolinska and Novo Nordisk.

Order application report "Insulin quantification: save sample and increase efficiency" here.

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